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4.4 EMS influences mitochondrial energy metabolism in GCs Because GCs are the nanny cells that feed the oocyte, mitochondria play a vital role in energy metabolism

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3) After IMAC, the eluted peak was immediately desalted (column: HiPrep 26/10, from GE healthcare) in buffer A: 10 mM NaP i at pH 8, 30 mM NaCl, 1 mM GSH, 10% glycerol, and 0.1% reduced Triton X-100

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However, most is produced in the liver when growth hormone levels increase in the blood