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Antibody-bound complexes were then captured by incubation with protein A Sepharose CL-4B beads (Amersham, 17-0963-03) for rabbit antibodies or protein G Sepharose 4B beads (Invitrogen, 101241) for mouse antibodies (both 20 l per 1 ml of lysate) for 4 h at 4 C

This process is considered one of the primary mechanisms of aging

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His wife seems more interested