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Antioxidant gene expression in brain regions of seals and mice via qPCR In order to test whether elevated antioxidant gene expression is also present in other brain regions and in diving mammals other than the hooded seal, we performed qPCR analyses in the visual cortex, the cerebellum and the hippocampus of hooded seals and harp seals, and compared results to mice (Figure 1)

Organoids were transferred to a 6 well-plate on a CO 2 resistant orbital shaker (Thermo Scientific) and grown in differentiation medium consisting of DMEM:F12 supplemented with 1X N2 supplement, 1X B27 supplement, 1X penicillin (100 units/mL)/streptomycin (100 g/mL), 0.1mM 2-mercaptoethanol, 1X NEAA, and 2.5 g/mL human insulin

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The participation of transition metal ions in the free radical generation is protected by several metal-sequestering proteins (e.g