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The membranes were blocked with 5 % skim milk (dilution in TBS with 0.05 % Tween 20 (TBST)) and incubated with primary anti-bodies (1:1000, dilution in TBST) for overnight at 4C

It also stimulates carbohydrate catabolism, protein anabolism, as well as lipid synthesis

Reducing concentrations of the protein is thought to have a beneficial effect on disease progress

If cause and severity are mild vitamin B12 deficiency may be managed via diet change or oral B12 supplements, especially if the cause of deficiency is poor intake

In the United States, the condition affects 6.4% of the population7.7% in women and 4.9% in men

Xiaochong Fan, [email protected] These authors share first authorship This article was submitted to Viral Immunology, a section of the journal Frontiers in Immunology Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers