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Differentiation of 3T3-L1 pre-adipocytes 3T3-L1 pre-adipocytes cells were cultured with standard culture media (DMEM, 4.5 g/L glucose, L-glutamine, sodium pyruvate, 10% FBS, 1% antibiotic-antimycotic solution) and grown for 48 h before initiating differentiation using the manufacturer's suggested protocol and modified from previous published work.[18] Briefly, standard culture media was supplemented with scheduled addition of adipogenic agents [3-isobutyl-1methyl xanthine (IBMX), Sigma Aldrich

For a side-by-side comparison of the two individual compounds, see: BPC-157 vs TB-500

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In addition to its effects on tissue repair and inflammation, BPC-157 has also been studied for its potential neuroprotective properties

3033 As the topical administration of human recombinant bFGF has been shown to be effective for wound healing in clinical situations, we selected bFGF as a positive reagent in this study