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We take the reaction to have standard bi-bi kinetics with K m = 97 M for APAP[24] and K m = 5

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18 Moreover, the 1,4-NQ glucosides rossoliside and plumbaside A, isolated from Nepenthes , showed no incorporation after feeding of either [U- 13 C 2 ]-sodium acetate or [U- 13 C 3 , 15 N]-alanine, suggesting these glycosides are storage forms with very low turnover rates

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NRPS Module Engineering Historically, a minimal module in an NRPS assembly line has been defined as C-A-T (condensation-adenylation-thiolation), and C-A didomains must be kept intact during engineering efforts to avoid perturbations to assembly line functionality