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No imidazole is required in the binding buffer, and 40 mM to 100 mM of imidazole can efficiently elute most target proteins

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(2) Raw cell lysate

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These approaches include combinatory administration of antibiotics, strengthening colonisation resistance of the gut microbiota to reduce VRE proliferation through commensals or probiotic bacteria, or switching to non-antibiotic bacterial killers, such as bacteriophages or bacteriocins