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Briefly, 4 10 4 H660 or 3 10 4 LNCaP cells were plated in complete growth media into each well of a 96-well Seahorse microplate and incubated overnight in 5% CO 2 at 37 C

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This is why supplementation is quite common, especially in specific groups of people

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Cell 13 , 11091121 (2002)
