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67: 4566-4579) as follows: Oligonucleotide primers HEV-140 (5'-TTCGGATCCATGGCGGTCGCTCCGGCC-3') (SEQ ID NO: 108) and HEV-141 (5'-TCAAGCTTATCATCATAGCACAGAGTGGGGGGC-3') (SEQ ID NO: 109) were used to clone a 1512 bp PCR-generated DNA fragment encoding HEV ORF2 amino acids 112 through 607 with its own ATG translation initiation codon and multiple stop codons from p61.2 into pCR2.1 (InVitrogen, San Diego, Calif.) by T/A PCR cloning

[link] Nishida T, Kubota S, Aoyama E, Yamanaka N, Lyons KM, Takigawa M., "Low-intensity pulsed ultrasound (LIPUS) treatment of cultured chondrocytes stimulates production of CCN family protein 2 (CCN2), a protein involved in the regeneration of articular cartilage: Mechanism underlying this stimulation", Osteoarthritis Cart 25: 759-769 (2017)

A 2020 retrospective cohort study (N = 4,072) of patients with gout found that urate-lowering treatment reduced the relative risks of coronary artery disease and stroke by ~30% compared with no treatment

Lactobacillus and Bifidobacterium species are the most commonly used probiotics

A few studies have also highlighted the role of bacterial, fungal, or viral infection in OSF

[DOI] [PMC free article] [PubMed] [Google Scholar] 93.Sacco R.L., Kasner S.E., Broderick J.P., Caplan L.R., Connors J.J., Culebras A., Elkind M.S., George M.G., Hamdan A.D., Higashida R.T., et al