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In humans, these latter mutations have often proved invaluable because, in addition to identifying genes of interest, they also provided specific mechanistic insights not possible by gene ablation

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[DOI] [PMC free article] [PubMed] [Google Scholar] 15.Vignais PV

It appears to act like a cellular glue, tightening the microscopic gaps between intestinal cells to prevent unwanted particles from leaking through

This is why IPL is sometimes called a photofacial rather than a laser treatment, the distinction is technical but meaningful

Cells were then washed twice with PBS and fixed in 4% PFA (Fisher Scientific 50-980-494) in PBS containing 0.1% Triton X-100 (PBST, Sigma-Aldrich T9284) for 20 min at room temperature at dark