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Adduct formation may also be a consequence of opioid binding to glutathione-like regions of aminergic receptors

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However, resistance to gemcitabine often develops within weeks of treatment, presenting a significant therapeutic challenge

Overall, 100 l of lysis buffer (1 mg/ml lysozyme, 10 mM Tris, and 1 mM EDTA, pH 8.0) was used to resuspend the cells and then incubated at 26C for 6 min