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After hydrolysis, 20 L of each sample was neutralized by the addition of 20 L of 6 M NaOH and 20 L of 0.2 mM HCl, and then filtered using a 0.45 m nylon syringe filter

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At therapeutic doses, acetaminophen is primarily metabolized in the liver through glucuronidation and sulfation into NON-TOXIC metabolites that can then be excreted in the urine