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Fifteen mg of HSS is loaded onto Superose 6 column (GE Healthcare) equilibrated in 20 mM Tri-HCl, pH 7.4, 50 mM KCl, 1 mM MgCl 2

For fermentative production, the cells were grown in SM1 minimal medium (1 g/L Trypton, 0.5 g/L Yeast extract, 100 M potassium phosphate buffer (pH 7.0), 75.7 M (NH 4 ) 2 SO 4 , 1.7 M NaCl, 1.0 M MgSO 4 , 0.1 M CaCl 2 , 20 mg/L ammonium ferric citrate (AFC), 0.6 M Na 2 MoO 4 , 40.4 M H 3 BO 3 , 2.9 M CoCl 2 , 1 M CuSO 4 , 8.1 M MnCl 2 , 3.04 mM Met) supplemented with 3% glucose

10.1074/jbc.RA119.007896 139 ToyokawaG.TakadaK.TagawaT.HamamotoR.YamadaY.ShimokawaM.et al (2019)

It seems that it has rarely been tested if the increase (or maintenance) of GSH levels is actually required for the rescuing effect afforded by NAC

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