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This method is utilized to measure AAs and carnitine/acetylcarnitines of type 2 diabetes patients, with results indicating that certain acetylcarnitine and AAs may be involved in the development of diabetic nephropathy

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L-carnitine is a quaternary amine which has different biological roles including: mitochondrial long-chain fatty acid oxidation, activation of aerobic glycolisis, enhancement of respiratory chain function, buffering of the mitochondrial acyl CoA/CoA couple, scavenger system for acyl groups peroxisomal fatty acid oxidation, branched amino acid metabolism, membrane stabilization, and donor of acetyl groups for biosynthesis 5

Koeth et al., 2019)