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Sample loading was perfomed at 800 bar with 100% buffer A (aqueous 0.1% formic acid) and eluted using a segmented gradient: 6% buffer B (acetonitrile 0.1% formic acid) for 1 minute, 623% B in 52 minutes, 2333% B in 7 minutes, 3390% B in 6 minutes, 90% B for 9 minutes

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10.1002/humu.23037 42 QuadrosE