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In such severe cases, cerebral oedema becomes clinically apparent 12 hours to 4 days after the overdose [18, 20, 50, 87], although CNS depression may be delayed if a slow-release preparation has been ingested [12, 50]

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difficile -associated mortality by decreasing the intestinal mucosal permeability in mouse models of CDI

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Worms were then washed with PBS to remove PFA, resuspended in 60% isopropanol, and incubated at room temperature for 15 min to dehydrate them
