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Potential cellular toxicity of DETA NONOate was determined by Cytotox Green staining using an IncuCyte live cell imager and analysis software (S3 2019, Sartorius) For experiments involving stable isotopic tracing, cells were incubated with media containing [U- 13 C]glucose, [1,2- 13 C]glucose or [5- 13 C]glutamine (Cambridge Isotopes), which replaces the unlabeled glucose or glutamine in regular media at the same concentration

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improves serum inflammatory markers and general health in subjects with overweight or prehypertension/mild hypertension: a randomized, double-blind, placebo-controlled trial

Therefore, as evidenced by our results, different types of anticoagulants may differentially stabilize and prevent spontaneous oxidation of free GSH in plasma (with suggestive evidence that EDTA better prevents spontaneous oxidation than heparin)