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Primers were obtained from Bio Basics

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Additional Molecular Feature database search settings: Use peaks with height 600, Modifiers: +H, +Na, +NH 4 , Mass match tolerance: 2.00 mDa, Extract separate MS/MS spectrum per collision energy: True

Johnstone TB, Gu Z, Yoshimura RF, Villegier AS, Hogenkamp DJ, Whittemore ER, et al

The reaction was terminated by the addition of 100 ng of trypsin inhibitor (type II-S from soybean, Sigma T-9128)

These networks allow for the exchange of ions, second messengers, metabolites, and amino acids from astrocytes to neurons