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To evaluate the dissolution and release properties of the suppositories, formulations containing 5%, 9%, and 12% water were separately placed in 5 mL of simulated intestinal fluid (SIF, MesGen, Tianjing, China)
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Subsequently, the concentration of GSSG was measured colorimetrically, employing a method similar to that used for total glutathione determination, 36 and calculated using a calibration curve for GSSG solutions
