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Less fat, more muscle leads to more burned calories
The resulting multivariate data were subjected to partial least squares discriminant analysis (PLS-DA), and differential metabolites were further explored through pathway analysis using MetaboAnalyst (www.metabonalyst.ca, accessed 6 June 2021)

IGF-1 LR3 contributes indirectly by preserving lean muscle mass during fat loss

A cGAS-mediated mechanism in naked mole-rats potentiates DNA repair and delays aging

Nuclear proteins are extracted from the pellet containing pure nuclear fraction, with the help of nuclear extraction buffer, which also has proteasome inhibitors to prevent nuclear protein degradation (Sundar and Tamizhselvi, 2020)