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MH, AB, AJM received funding from BERG for the project

10.1016/j.neuron.2010.12.003 161 NunesG
For example, many soluble globular proteins precipitate if the pH of the solution is set at the pI of the protein
This staggered treatment strategy may be clinically beneficial in maximizing efficacy while minimizing the potential increase in toxicity that might be anticipated from simultaneous co-treatment with two agents

The slides were rinsed with PBS, incubated in permeabilization solution (0.1% Triton X-100, 0.1% sodium citrate) for 2 min on ice and rinsed twice with PBS
*Note: The NAD IV Therapy has a potentially longer run time of 3-4 hrs
