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Cloning and recombination of plasmids and fragments were performed using the One Step Cloning Kit provided by Vazyme (#C112, Nanjing, Jiangsu, China)

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DMSO (Dimethyl Sulfoxide) DMSO is widely used for peptides that exhibit significant hydrophobicity

Namely, it is always applied alone (Sikiric et al., 1993

The regulatory distinction you made is so crucial: the July FDA advisory committee meeting on BPC-157, TB-500, KPV, and others is about compounding access through the 503A pathway, not approval, and not evidence of efficacy

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