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( N, O ) Representative images ( N ) and quantification ( O ) of fold-change in maximum CHMP4B-mNG puncta in 293 T cells upon treatment with vehicle control (DMSO) or 0.25 mM LLOMe and following knockdown with shRNAs against Luc or TFG and reconstituted with wild-type TFG or TFG lacking the PB1 or coiled-coil (CC) domains

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Buffer composition : Must maintain pH, ionic strength, and prevent aggregation