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10.1002/advs.202307388 79 JosseJ.VelardF.GangloffS

Neurobiol Aging 28(9):14361445

Briefly, 10L of each sample was diluted in 90 L 1X Assay Buffer and transferred to a Nunc 96-Well Polypropylene MicroWell Plate (Thermo Scientific Nunc, Roskilde, Denmark)

Ensure the vials remain sterile before use

A common approach is 2-3 mL BAC water per 10 mg vial

The observation that patients with AD carrying two alleles of the APOE4 risk variant have approximately half the cerebrospinal fluid (CSF) melatonin levels compared to those with only one allele of this variant [75] could suggests that APOE4/4 carriers might particularly benefit from supplementing with exogenous melatonin