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Individualization should consider renal function, metabolic demand, and concurrent protein sources

Since a loss of DAPI signal is also observed with nitrofurantoin, which does not bind to DNA but disperses the nucleoid in a similar manner as Cu 3 -TC-Py, nucleoid fragmentation is a considerably more likely explanation for the dispersed DAPI signal and the loss of protein localisation than competition between DAPI and Cu 3 -TC-Py for the minor groove, although it cannot be excluded that the latter may partially contribute to the reduced DAPI signal

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