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the lysate was clarified by centrifugation at 15,000 nx g for 15 min, protein concentrations were determined using the BCA total protein assay (Pierce), and then appropriate volumes of the lysates were diluted with 2 reducing Laemmli buffer and heated for ten min at 95 C

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In the 1960-1970s, developed western countries started to use activated carbon technology in potable water treatment to enhance the removal of organic contaminants

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Fluoride Fluoride is a thyroid-suppressing halogen found in our water, toothpaste, and even black and green tea

10.1097/brs.0b013e3181a3967f 2 AkedaK.OhishiK.TakegamiN.SudoT.YamadaJ.FujiwaraT.et al (2022)