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These results are supported by studies demonstrating that p28 binds to the p53 DBD through non-polar residues at the interface, creating a short-range hydrophobic interaction

PLoS ONE 7(10):e46214

Thiol groups were subjected to derivatization by mixing 250 l of the filtered homogenate with 45 l of 200 mM HEPES, pH = 8.26.3 mM DTPA, and 1 l of monobromobimane (Invitrogen, Eugene, OR, United States), and the solution was incubated at room temperature in darkness for 30 min

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