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Protein lysates (30 g) were separated in 10% or 15% SDS-PAGE and transferred onto Immobilon-P PVDF membrane (Sigma-Aldrich)

5 Multilevel regulatory mechanisms of the de novo serine synthesis pathway in malignant tumors 5.1 Transcription factor regulation Multiple key transcription factors have been verified to directly regulate the expression of core enzymes in the SSP

Methylation-specific PCR MSP is a sensitive, cost-effective, and efficient method for detecting DNA methylation, making it suitable for small-scale analyses (61, 62)

Combining peptide enhancement with relationship enrichment produces optimal results

The modulation in the expression and redundancy of GSTs ascertain its enigmatic roles in plant growth and development

Kit Components Additional Materials Required Microplate reader capable of measuring absorbance at 405 nm Precision pipettes to deliver 2 L to 1 mL volumes Multi-channel pipettes to deliver 20 L to 200 L volumes Tubes to prepare sample dilutions Ultrapure water 50 mL conical tubes Protocatechuic acid (HClO 4 ) (PCA) Potassium hydroxide (KOH) Assay Procedure Summary Add 50 L of the diluted GSSG Standards or unknown samples to the 96-well microtiter plate