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Description
Cancer 130, 450456 (2024)

The remaining iodoacetamide was quenched by adding 5 mM DTT and the proteins were digested with 200 ng trypsin (Trypsin Gold, Promega) at 37 C ON

To achieve the desirable therapeutic effect, the reservoir and the multi-laminate type were considered the best choices

Extracted samples were vortexed at 4 C for 10 min, cleared by centrifugation (17,000 g , 10 min, 4 C) and then supernatants were analysed by LCMS

Nat Rev Drug Discov 19(12):884901

A375 and A375-S2 human skin melanoma cells demonstrated anti-proliferation in a dose-dependent manner, indicating that biological substances were used and had anticancer properties [130]
