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Habituation is done prior to the test where the mouse is placed in the central chamber and is free to explore the 8ARM and find the reward

doi: 10.1161/CIRCRESAHA.108.184457

V., Popuchiev, V

PKR, Protein Kinase R

www.nature.com , Nature Neuroscience , vol

To evaluate this property, we systematically characterized its biodegradation behavior in PBS at pH 5.4 (simulating the lysosomal compartments), pH 6.5 (mimicking the OA pathological microenvironment), and pH 7.4 (representing normal physiological conditions)
