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Include the following controls on every assay plate: Blank control (reagent only, no cells): establishes background luminescence baseline

In addition, oligodendrocytes can mediate intracellular calcium overload through P2X7 receptors during cerebral ischemia, Calcium overload mediates glutamate and ATP excitotoxicity in oligodendrocytes, promoting ROS generation (Domercq et al

O-GlcNAcylation destabilizes the active tetrameric PKM2 to promote the Warburg effect

Activation of GPER1 has been associated with vasodilation, reduced blood pressure, and protection against vascular injury

In addition, a recent study suggests that Suanzaoren decoction modulates the DJ-1/Nrf2 signaling pathway, alleviating neuronal loss, synaptic damage, and ferroptosis associated with Alzheimers disease

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