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Oxidation of acrolein may precede conjugation with GSH, thus forming acrylic acid

A survey scan at 70,000 resolution scanning mass/charge ( m / z ) of 3501600 was performed with an automatic gain control target of 1e 6 with a maximum injection time (IT) of 65 msec, then a high-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) at 37 NCE (normalized collision energy) of the 11 highest intensity ions at 17,5000 resolution, 1.5m/z isolation width, 5e 4 AGC, and 65msec maximum IT

E1E2, E2E1 or E2E1-I5350A were co-expressed with a plasmid expressing furin in a 1:1 ratio to ensure E1/E2 cleavage

PMGs contain a unique profile of nucleotides and peptides from veal bone

In addition, we conducted comparative studies based on the histological score (Table 2)

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