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[DOI] [PMC free article] [PubMed] [Google Scholar] 120.Silva-Garca O., Valdez-Alarcn J.J., Baizabal-Aguirre V.M

Calibration curves were built with 23 of the BA reference compounds and detected in single ion recording (SIR) mode on mass spectrometer

The supernatants were incubated at 4 C for 30 min with Ni-NTA resin (ThermoFisher Scientific), which had been equilibrated with 2.5 ml of wash buffer 1 (20 mM HEPES, 500 mM NaCl, 10 mM Imidazole, 10% glycerol, 0.5 mM TCEP, pH 7.5)

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He died of pleurisy, December 14th, 1782, aged nearly 42