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The tissue was incubated at 37 C on a shaking incubator set at 250 rpm for 1 h, with the centrifuge tube being gently inverted every 20 min

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These borderlands are sites of tension, particularly for disadvantaged youth who seek to make good on the promise of the free market (Comaroff and Comaroff 2000, 308)

Acknowledgments The authors thank all people with ME/CFS for participating in the study, research nurses and clinical staff from Clinical Research Support Unit (USIC) at the Vall d'Hebron Research Institute (Barcelona, Spain) for their assistance with informed consent, blood sample collection, and study protocol coordination