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19 Cook-MillsJ

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Furthermore, melatonin indirectly combats free radicals by chelating metal ions in the brain [148]

In a separate analysis in neuronal cells, overexpression of GPx-1 and other antioxidant enzymes also blocks AIF-translocation from mitochondria to the nucleus in a model of ischemia-induced apoptosis (404), suggesting that intracellular oxidants can promote AIF transmigration

Nickel affinity chromatography is a technique used to purify proteins that contain a polyhistidine tag (his-tag)

Further information can be found in the approximatively 10 g of EV protein lysates were solubilized in Laemli buffer, resolved by a 420% Criterion TrisHCl gel electrophoresis system (Biorad, Hercules, CA, USA) and transferred to a 0.2 m nitrocellulose membrane (Biorad, Hercules, CA, USA)