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Determination of IL-1, IL-17, IL-4 and BDNF Expression Cytokines IL-1, IL-17 and IL-4, as well as BDNF, were assessed in the homogenized PFC and hippocampus of the mice using commercial kits (R&D Systems, Billings, MT, USA) by Enzyme-Linked Immunosorbent Assay (ELISA) method, with a detection limit for each cytokine of 5 pg/mL
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