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Glutathione concentration determined by monochlorobimane assay Stock solutions of assay dye (monochlorobimane (MCB), 22 mM) and glutathione- S -transferase (50 units/mL) were prepared in PBS and stored protected from light at 20 C

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(PubMed) Xue YZ, Wang LX, Liu HZ, Qi XW, Wang XH, Ren HZ
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For experiments involving intracellular staining of cytokines, T cells were stimulated for 3 h (mouse) and 6 h (human) with phorbol 12-myristate 13-acetate (PMA
