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The overexpression of metrnl induces the expression of SIRT3 by promoting the expression of transcriptional activator PGC-1, increasing the expression level of UCP1 and the activation of AMPK in PA-treated RTECs to accelerate mitochondrial autophagy, maintain mitochondrial homeostasis, and alleviate lipid accumulation (a thermogenic factor) (Zhou et al., 2023)

Avoid vigorous shaking, which can introduce air bubbles

Metabolomics activity screening for identifying metabolites that modulate phenotype

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Once reconstituted, the solution should be used promptly or aliquoted and stored at -20C to avoid repeated freeze-thaw cycles, which can degrade the compound

2.1 Multi-target cardiovascular benefits of TCM natural compounds Natural metabolites from TCM exhibit multifaceted cardioprotective properties that set them apart from single-target conventional drugs