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Cbh was expressed and purified through immobilised metal ion affinity chromatography (IMAC) with nickel-charged agarose resins followed by size exclusion chromatography on a HiLoad 16/600 Superdex 75 pg column (GE Healthcare Life Sciences)

1-deoxynojirimycin, an inhibitor of intestinal -glucosidase, can effectively inhibit the conversion of glucose in the human body and is superior to the -glucosidase inhibitor acarbose in terms of absorption (61)