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Hypogonadism is characterized by low levels of testosterone due to disruption of the HPG axis

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Materials and Methods Chemicals Aspirin (acetylsalicylic acid, ASA), cytochrome c, reduced and oxidized glutathione, (GSH and GSSG), 1-chloro 2,4-dinitrobenzene (CDNB), cumene hydroperoxide, glutathione reductase, 5,5-dithio bis-2-nitrobenzoic acid (DTNB), 2-thiobarbituric acid, buthionine sulfoximine (BSO), N-acetylcysteine (NAC), NADH, NADPH, coenzyme Q2, antimycin A, dodecyl maltoside and ATP bioluminescent somatic cell assay kit were purchased from Sigma-Aldrich Fine Chemicals (St Louis, MO, USA)

(4) Most of what a total B12 assay measures is bound to haptocorrin and is not available for uptake by most cells
