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10.1159/000335798 92 NickelsJ

Analytical size-exclusion chromatography ZSWIM8ELOB/C, ZSWIM8 mono ELOB/C and ZSWIM8 dimer ELOB/C (10 M) were each incubated with CUL3RBX1 (12 M) in 25 mM HEPES, 150 mM NaCl and 1 mM TCEP, pH 7.5, at 4 C for 30 min before analysis by size-exclusion chromatography (Superose 6, Cytiva)

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also observed similar phenomena in the testes of STZ-induced diabetic mice and HG-treated GC-1spg cells, and found that BRD7 inhibits Clusterin expression by regulating hypermethylation of the Clusterin promoter in an EZH2-dependent manner, thereby suppressing AMPK signaling to promote ferroptosis and induce DM-related testicular injury [178]

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Thus, by acting directly and indirectly via inducing AP-1, NFB is important in the expression of rat GCLC and perhaps human GCLC as well