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Advantages and disadvantages when using one-step versus two-step assays in RT-qPCR Reverse transcription in RT-qPCR Choosing total RNA vs mRNA When designing a RT-qPCR assay it is important to decide whether to use total RNA or mRNA as the template for reverse transcription
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Wrobel JK, Seelbach MJ, Chen L, Power RF, Toborek M

however, it also reduces metal ions that generate free radicals through the Fenton reaction [25]
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