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[DOI] [PMC free article] [PubMed] [Google Scholar] 121.Govindarajan A., Rao B.S.S., Nair D., Trinh M., Mawjee N., Tonegawa S., Chattarji S

The polyhistidine affinity tag or His tag consists of a variable number of consecutive histidine residues (usually six) that coordinate, via the histidine imidazole ring, transition metal ions such as Ni 2 + or Co 2 + immobilized on beads or a resin for IMAC (Gaberc-Porekar and Menart, 2001

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But as we'll explore, 'cold' can mean different things, and the choice between refrigeration and freezing has significant implications for your Glutathione storage strategy

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