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Then, the sections were covered with ice-cold 100% methanol for 10 min at 20 C, washed three times, and blocked with 0.5% casein in PBS buffer for 1 h at room temperature

doi: 10.1124/jpet.108.149211

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PG is one of the commonly occurring species of Pulicaria in Iran, locally known as Alaf-e Heiyze or Kak kosh. It is a perennial or subshrub that primarily grows in temperate biomes and is native to Iraq, Central Asia, the Western Himalayas, and the Arabian Peninsula [104]