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Exosome detection via surface-enhanced raman spectroscopy for cancer diagnosis

doi:10.1007/s00018-013-1514-y

The mice were acclimatized to laboratory conditions for 1 week prior to experimentation

The juice samples were first acidified with a 2% metaphosphoric acid solution to stabilize vitamin C and precipitate interfering proteins

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Subsequently, the aspartateglutamate antiporter (encoded by SLC25A13 gene) exports aspartate into the cytosol where cytosolic glutamic-oxaloacetic transaminase (GOT1) transfers an amino group from aspartate to ketoglutarate to produce glutamate and oxaloacetate to complete the cycle, which is, in turn, reduced to malate in conjunction with oxidizing NADH to NAD + by cytosolic MDH (Fig