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10.1136/gut.2005.066423 [DOI] [PMC free article] [PubMed] [Google Scholar] 53.Matsumoto K, Appiah-Pippim J, Schleimer RP, Bickel CA, Beck LA, Bochner BS

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Fix: Aim the needle at the inside wall of the vial

coli BL21(DE3) cells and cultured in LB medium at 37 C until the optical density at 600 nm (OD) reached 0.50.6