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Polymeric micelles for the delivery of poorly soluble drugs: From nanoformulation to clinical approval
By contrast, the protein purity by IMAC is relatively crude, a time-consuming second purification step, e.g., ion exchange chromatography is essential for sensitive applications

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Pellets were grinded with pestles in 100 l of the same buffer, cells were lysed by two freeze-thaw cycles and insoluble material was removed by filtration in a COSTAR R Spin-X centrifuge tube filter
