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K562 cells were collected and centrifuged at 3000 rpm for 6 min in a culture medium, washed once with hybridization buffer (pH 7.4) and then spun down at 3000 rpm for 6 min

Nature 537:185190 Kruegel AC, Gassaway MM, Kapoor A, Vradi A, Majumdar S, Filizola M, Javitch JA, Sames D (2016) Synthetic and receptor signaling explorations of the mitragyna alkaloids: mitragynine as an atypical molecular framework for opioid receptor modulators

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doi:10.4103/jhrs.JHRS_74_16 Vijayakumar A, Kim EK, Kim H, Choi YJ, Huh KB, Chang N

Active cancer or history of malignancy Pregnancy or breastfeeding Known peptide allergies Athletes subject to anti-doping testing (WADA prohibited) Raynauds disease or phenomenon Cold urticaria (hives from cold) Uncontrolled hypertension Peripheral vascular disease Open wounds (for local application) Pregnancy Cardiovascular conditions Diabetes (especially with neuropathy) Current infections Immunosuppression Blood clotting disorders Quality control issues represent perhaps the greatest practical risk with BPC-157