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Additionally, chemoaffinity protocols such as immobilized metal affinity chromatography (IMAC) or titanium dioxide (TiO 2 ), although widely used in phosphoproteomics, lack site selectivity for phosphorylation at serine (pS), threonine (pT) or tyrosine (pY) 8,9 and exhibit a sequence bias in favor of peptides rich in aspartic (D) and glutamic (E) acid

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Cell 115:97108

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