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Once the samples reached confluency the cells were subcultured and all tendon fibroblasts between passages 2 and 4 that had a proper growth rate and normal fibroblast shape were used in the experimentation process

This secretory profile contributes to chronic inflammation, which is a hallmark of aging and a driver of multiple diseases

[PMC free article: PMC209498] [PubMed: 11602619] 33

However, no differences were observed in the percentage of distance traveled in the center zone, a common measure of anxiety-like behavior, indicating that HDAC9 deletion in CA1 does not affect anxiety levels (Fig

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The reaction is local, not systemic